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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was located to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant purpose in marketing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however readily available.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.
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This guide outlines more doable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make sure the best possible final results are...
Antibodies bound to the blocking peptide no longer bind to the epitope around the target protein. By comparing the staining from your blocked antibody as opposed to the antibody by itself, one can see which staining is distinct.
Goal Information Cyclin E belongs on the remarkably conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance in the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins exhibit distinctive expression and degradation designs which lead into the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates at the G1-S phase boundary and is degraded as cells development by S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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